Enhancement of extracellular laccase production from Lenzites elegans KSG32: Taguchi orthogonal array experimental design methodology

  Kiran Lakshmi M S,  Aiswarya C,  Nayana P,  Prasanta K Dash,  Padma Nambisan

Fungal extracellular laccases play a pre-eminent role in biotechnology applications due to their high redox potential and wide substrate specificity. For any industrial applications large quantity of enzyme is needed and must be produced economically. The present study focused on the enhancement of laccase production by Lenzites elegans KSG32 under solid-state fermentation method using Taguchi orthogonal array experimental design methodology. An L8 orthogonal array was designed using Qualitek-4 software to study the effect of seven factors selected on the basis of one factor at a time approach. The interactions and influences between the factors were studied. The predicted optimum conditions for the production were: incubation period (3 days), sugarcane bagasse size (0.02 m), pH (3), maltose concentration (2.5 %), beef extract concentration (0.1 %) and pyrogallol concentration (0.25 mM). Tween 20 was identified as unnecessary media component and excluded. After optimization of SSF conditions laccase production was increased by 39.74 %. The results suggest that both the design method and strain has promising potential in the production of laccases for biotechnological applications like synthetic dye decolourization.

Keywords- Taguchi method, Design of experiments, Optimization, Laccase, Lenzites elegans KSG32, Solid state fermentation

Unique Identification Number - IJEDR1802064
Page Number(s) - 369-374
Pubished in - Volume 6 | Issue 2 | April 2018
DOI (Digital Object Identifier) -   
Publisher - IJEDR (ISSN - 2321-9939)

  Kiran Lakshmi M S,  Aiswarya C,  Nayana P,  Prasanta K Dash,  Padma Nambisan,   "Enhancement of extracellular laccase production from Lenzites elegans KSG32: Taguchi orthogonal array experimental design methodology", International Journal of Engineering Development and Research (IJEDR), ISSN:2321-9939, Volume.6, Issue 2, pp.369-374, April 2018, Available at :http://www.ijedr.org/papers/IJEDR1802064.pdf